Hiseq sequencer has outstanding advantages such as high precision, high throughput, high sensitivity and low operation cost. A new sequencing technology based on sequencing-by-synthesis (SBS). The bridge PCR reaction is realized on a small chip (flowcell) through a single molecular array. Through reversible blocking technology, only one base is synthesized at a time, and then the corresponding laser is used to excite the fluorescent group to capture the excitation light, so as to read the base information. It can complete the research of traditional genomics (sequencing and annotation) and functional genomics (gene expression and regulation, gene function, protein / nucleic acid interaction) at the same time.
(Edited by Ruanhong Cai)