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High field Nuclear magnetic resonance spectroscopy

Progress in the understanding of the chemical structures of DOM has been impeded by lack of a suitable method for its isolation and its intrinsic chemical complexity. Nuclear magnetic resonance (NMR) spectroscopy is a valuable tool for elucidating the structures of natural organic matter such as marine DOM. Advanced solid-state NMR techniques have developed and applied for characterizing complex DOM samples. This new, systematic protocol can identify many more functional groups by selectively retaining certain peaks and eliminating others by two-dimensional 1H–13C NMR. Overall, the high field NMR (≥ 800MHz) provides unprecedented resolution in resolving functional groups of natural complex DOM, thus favoring understanding of RDOC in the ocean.

(Edited by Ruanhong Cai)